Remco Swart

Nano LC is now widely applied in proteomics, and also is robust enough for application in fields such as pharmaceutical analysis.

An optimization strategy to obtain the best possible performance in the shortest analysis time for comprehensive off-line 2D-LC.

In HPLC method development, screening of various para-meters such as stationary phase, eluents, and temperatures is conducted to find optimal resolution. However, method development can be a time consuming and inefficient process. UHPLC technology can be applied to significantly shorten both the analysis and development times. Here we describe an integrated and ultrafast automated method scouting solution that provides fast and efficient method development processes.

Multidimensional liquid chromatography (MDLC) techniques are essential for the separation of highly complex proteomic samples. Advantages of off-line MDLC techniques over on-line approaches include high flexibility in choice of column dimensions and mobile-phase compositions, and the ability to reanalyse sample fractions. Here we present a fully automated off-line two-dimensional chromatographic approach for the analysis of proteomic samples using an UltiMate 3000 system optimized for proteomics MDLC.

Polystyrene-divinylbenzene (PS-DVB) trap columns have been evaluated for microcolumn switching applications. In contrast to traditional stationary phases, which consist of packed particles, the monolithic separation medium is made of a continuous, rigid polymeric rod with a porous structure. The absence of intraparticular void volume increases separation efficiency, allowing for faster separations. Column lifetime is higher compared to packed columns.