In this study, we aimed to use a charge modulated HILIC stationary phase (iHILIC®-Fusion) to separate different siderophores of the Pseudomonas taiwanensis VLB120 bacteria.
Phospholipids (PLs) are the major components of cellular membranes. They are important for the functionality of membrane proteins or serve as precursors for second messengers. Several studies reveal the role of PL alterations in various diseases such as cancer (1). Therefore, it is crucial to identify and quantify PLs in complex biological samples for lipidomic studies and clinical research.
Hydrophilic interaction liquid chromatography coupled to electrospray ionization mass spectrometry (HILIC–ESI-MS) has been established as a method to separate and quantify polar and ionic analytes in a direct way for two decades. HILIC separation is based on the polarity of analytes, so the more polar analytes have stronger retention on a HILIC column.