LCGC North America
Protein unfolding and aggregation can be serious considerations when designing laboratory and preparative chromatographic purification steps. This problem has been studied most thoroughly within the contexts of reversed-phase chromatography and hydrophobic interaction chromatography. However, there are currently no robust methods for resin selection capable of predicting adsorbed-phase protein stability as a function of amino acid sequence, secondary or tertiary structure, or resin characteristics.
