Jelle De Vos | Authors

The last decade has witnessed how liquid chromatography columns and instruments changed from long bulky columns with relatively large fully porous particles operated at modest pressures (100Ð200 bar), to short compact columns with small superficially porous particles operated at ultrahigh pressures (1200Ð1500 bar). This (r)evolution has resulted in a tremendous increase in achievable separation performance or decrease in analysis time, but requires a good knowledge of optimal chromatographic conditions for each separation problem and, concomitant, the right instrument configuration.

The last decade has witnessed how liquid chromatography columns and instruments changed from long bulky columns with relatively large fully porous particles operated at modest pressures (100–200 bar), to short compact columns with small superficially porous particles operated at ultrahigh pressures (1200–1500 bar). This (r)evolution has resulted in a tremendous increase in achievable separation performance or decrease in analysis time, but requires a good knowledge of optimal chromatographic conditions for each separation problem and, concomitant, the right instrument configuration.