Ken Broeckhoven | Authors

Articles

Where Has My Efficiency Gone? Impacts of Extracolumn Peak Broadening on Performance, Part 4: Gradient Elution, Flow Splitting, and a Holistic View

In this instalment, we focus on the impact of elution mode (isocratic or gradient) and postcolumn flow splitting on the total level of extracolumn dispersion (ECD) in a liquid chromatography (LC) system, and demonstrate the use of a free, web-based calculator that can be used to guide decision making aimed at reducing ECD during method development.