Gert Desmet | Authors

An introduction from guest editor, Gert Desmet from Vrije Universiteit Brussel, Belgium

In this extended special feature to celebrate the 35th anniversary edition of LCGC Europe, leading figures from the separation science community explore contemporary trends in separation science and identify possible future developments.

In this extended special feature to celebrate the 35th anniversary edition of LCGC Europe, key opinion leaders from the separation science community explore contemporary trends in separation science and identify possible future developments.

An introduction from the guest editor of this special supplement from LCGC Europe revealing recent developments in high performance liquid chromatography (HPLC) and ultrahigh-pressure liquid chromatography (UHPLC).

The structural complexity of monoclonal antibodies (mAbs) challenges the capabilities of even the most advanced chromatography and mass spectrometry techniques. This study examines the use of micro-pillar array columns in combination with mass spectrometry for peptide mapping of both mAbs and antibody–drug conjugates (ADCs).

The last decade has witnessed how liquid chromatography columns and instruments changed from long bulky columns with relatively large fully porous particles operated at modest pressures (100Ð200 bar), to short compact columns with small superficially porous particles operated at ultrahigh pressures (1200Ð1500 bar). This (r)evolution has resulted in a tremendous increase in achievable separation performance or decrease in analysis time, but requires a good knowledge of optimal chromatographic conditions for each separation problem and, concomitant, the right instrument configuration.