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© 2022 MJH Life Sciences™ and Chromatography Online. All rights reserved.
Flow-through immobilized-enzyme reactors (IMERs) can streamline protein digestion for bottom- and middle-up LC–MS characterization while providing high accuracy and reproducibility.
Leading biopharmaceutical companies are integrating HPLC into production processes for monitoring critical quality attributes (CQAs) with high-resolution mass spectrometry (HRMS) detection.
The potential of multidimensional online peptide mapping analysis as a strategy for improving a postlabeling workflow for protein–protein interactions is demonstrated using both hydroxy radical footprinting–mass spectrometry (HRF–MS) and LC–MS/MS.