Davy Guillarme | Authors

Multi-isocratic elution mode is a powerful chromatographic approach for characterizing minor isoforms of monoclonal antibodies.

In the second part of this review of the current state of HIC, some practical considerations are explained, including method development, selection of the phase system, combined salt systems, and possibilities to combine HIC with other chromatographic modes.

A review of the current state of HIC, focusing on retention and separation mechanisms with the aim of developing more robust methods. Can HIC be considered as a non-denaturing and non-destructive technique with advantages for protein analysis?

A new free simulator is available for students, educators, and trainers to teach and perform virtual HPLC experiments that are applicable to real HPLC instrumentation and method development.

An introduction from guest editors, Koen Sandra and Davy Guillarme, on this special supple-ment from LCGC Europe focusing on recent developments in biopharmaceutical analysis.

The potential of multidimensional online peptide mapping analysis as a strategy for improving a postlabeling workflow for protein–protein interactions is demonstrated using both hydroxy radical footprinting–mass spectrometry (HRF–MS) and LC–MS/MS.