Kevin D. Altria

This article discusses the role of CE for vitamin analysis and highlights a number of practical applications.

A review of the role of CE for analysing organic acids

The use of dynamic coating systems/approaches in CE can result in improved routine performance and/or selectivity.

Why is method repeatability so important in capillary electrophoresis?

CE is suitable for the analysis of simple inorganic ions, such as chloride, nitrate and sulphate. This method is robust and rugged.

The benefits of using capillary electrophoresis to detect metal ions.

A review of the expanding range of consumables available to capillary electrophoresists and why they are useful

Describing the theoretical background on how the separations occur, the operating approaches that can be taken and method development.

The ionic strength of a sample and the solvent used to dissolve it play a pivotal role in routine capillary electrophoresis (CE).

Microemulsion-based HPLC (MELC) is a recent development offering reduced sample preparation times for complex samples and generic separation conditions applicable to a wide range of solutes. This article introduces the concepts of MELC and discusses the possible benefits and future applications.

Microemulsion liquid chromatography (MELC) is a recent development offering reduced sample preparation times for complex samples and generic separation conditions applicable to a wide range of solutes. This article introduces the concepts of MELC and discusses the possible benefits and future applications.

Sample diluent ionic strength, pH, organic solvent composition and viscosity can all have a significant impact on the performance of CE separation. It is, therefore, essential that the choice of solvent is optimized during method development. The optimized diluent should be fully specified in the method and used consistently in method validation and all subsequent applications of the method.

Accurate preparation and correct selection of buffers is essential to obtain reproducible and consistent results in capillary electrophoresis (CE). A number of factors should be considered in buffer optimization, including the pKa of the buffering ion and the analyte as well as the molarity of the acid or base used in the adjusting procedure. Accurate recording of the precise reagents used and the procedures performed is necessary to prepare buffers consistently.

Accurate preparation and correct selection of buffers is essential to obtain reproducible and consistent results in capillary electrophoresis (CE). A number of factors should be considered in buffer optimization, including the pKa of the buffering ion and the analyte as well as the molarity of the acid or base used in the adjusting procedure. Accurate recording of the precise reagents used and the procedures performed is necessary to prepare buffers consistently. "Poor reproducibility of results and poor quantitative precision will be attainable in CE assays without significant attention being paid to the preparation of buffers used."

This "CE Currents" column examines the use of capillary electrophoresis (CE) in three important areas of pharmaceutical analysis.

Column Editor Kevin Altria explores new websites and shares information about the features of these sites.

By adopting standard buffers, analysts can gain a great deal of operating efficiency and significantly reduce method development and validation costs.