Lee Williams | Authors

A biennial meeting jointly organized by the Environmental Chemistry Group, Separation Sciences Group, and the Water Science Forum, and discussing the latest advances in the analysis of complex environmental matrices, is now in its eighth year. The most recent iteration of the event occurred on Friday 22 February 2019 in the Science Suite, Royal Society of Chemistry, Burlington House, in London, UK. This meeting review offers an overview of what is happening in the industry.

Biotage GB Ltd

In this application, we demonstrate the use of supported liquid extraction (SLE) for the extraction of beta blockers and NSAIDS from plasma compared with traditional liquid–liquid extraction. SLE was demonstrated to yield consistent LOQs using lower sample volumes.

The use of EVOLUTE CX mixed-mode columns provides high and consistent recoveries across a range of complex matrices, from biological fluids to milk products. The dual retention mechanism ensures that matrix components such as proteins, salts and phospholipids are removed, resulting in lower ion suppression and accurate analysis.

Sample preparation is an essential technique to remove unwanted matrix components prior to LC–MS-MS analysis of drugs in biological fluids. Plasma matrix components whether endogenous (salts, proteins, and phospholipids) or exogenous (dosing vehicles, e.g. PEG 400), can interfere with compounds of interest leading to regions of ion suppression or enhancement. This can lead to inaccurate quantitation and have adverse effects on sensitivity. Mixed-mode SPE provides cleaner extracts as a result of rigorous interference wash steps, afforded by the dual retention mechanism of the sorbents.

Sample preparation is an essential technique to remove unwanted matrix components prior to LC–MS-MS analysis of drugs in biological fluids. Plasma matrix components whether endogenous (salts, proteins, and phospholipids) or exogenous (dosing vehicles, e.g. PEG 400), can interfere with compounds of interest leading to regions of ion suppression or enhancement. This can lead to inaccurate quantitation and have adverse effects on sensitivity. Mixed-mode SPE provides cleaner extracts as a result of rigorous interference wash steps, afforded by the dual retention mechanism of the sorbents.