Guest columnist Matt Przybyciel reviews the structure, behaviour and applications of both alkyl- and phenyl-fluorinated phases in HPLC. The author presents some guidelines on potential areas of application for alkyl and phenyl-fluorinated phases, in which conventional C8, C18 and phenyl phases might have insufficient selectivity or too strong retention.
Continued from last month, this month's column discusses specially designed columns for use in highly aqueous environments. Many of these columns also can be used as regular reversed-phase columns for a range of mobile phases.
The authors suggest ways to avoid phase collapse and regenerate collapsed phases.