A recent study developed and validated a highly sensitive reversed-phase high-performance liquid chromatography (RP-HPLC) fluorescence method using OPA/ethanethiol derivatization to simultaneously quantify multiple gentamicin C-subtypes in tiny murine perilymph samples, enabling improved pharmacokinetic analysis in the inner ear. LCGC International spoke to Shreshtha Dash and Peter Streyger, two of the authors of the paper that resulted from this study,1 about their work.