Siji Joseph | Authors


Simultaneous Determination of Methotrexate and Sulfasalazine in Plasma by HPLC–DAD

Methotrexate and sulfasalazine are often used together to treat rheumatoid arthritis, so it is valuable to be able to monitor plasma levels of both drugs simultaneously. This method achieves that by using two detector wavelengths, 304 nm for methotrexate and 358 nm for sulfasalazine.