Tecan

Optimizing sample preparation is critical for generating high-quality, reproducible analytical data, particularly when working with complex matrices. Yet, it often demands significant time and effort. Traditional methods, including solid phase extraction (SPE), supported liquid extraction (SLE), filtration, and nucleic acid (NA) extraction, rely on column- or plate-based protocols and can face limitations in throughput, reproducibility, and the handling of difficult samples. Conventional approaches such as centrifugation and vacuum manifolds introduce further challenges, from added manual intervention and inconsistent flow to difficulty processing viscous samples. Positive pressure instrumentation uses controlled gas flow to push liquids through columns or plates, maintaining constant pressure across all positions to improve analyte recovery, reduce matrix effects, and support automation. This whitepaper examines how positive pressure compares to conventional methods and how Tecan's Resolvex platforms apply the technology across manual, semi-automated, and fully automated workflows.

This application note explores the use of positive pressure processing for plasmid DNA extraction by automating key preparation steps in a large-volume purification workflow. Performed in a compact benchtop format, this automated approach helps improve consistency, reduce hands-on time and streamline plasmid DNA purification for molecular biology and genomics applications.

This application note demonstrates an automated workflow for the analysis of trypsin-digested bovine serum albumin (BSA), combining on-column digestion and peptide clean-up using solid phase extraction (SPE) within a single column. Using automated positive pressure processing, the workflow streamlines sample preparation, reduces hands-on time and delivers consistent, reproducible peptide recovery for downstream analysis.

This poster highlights an enhanced workflow for automated monoclonal antibody (mAb) purification using positive pressure processing with AffinEx™ Protein A SPE columns ranging from 1 ml to 50 ml. The study evaluates purification performance across multiple column sizes, demonstrating a scalable approach that helps laboratories improve throughput, maintain consistency and streamline mAb purification from development through larger-scale applications.

This webinar recording provides a practical introduction to positive pressure processing for sample preparation, highlighting its advantages in achieving more consistent flow rates, improving reproducibility and reducing variability across samples. Learn how positive pressure technology can support reliable, high-quality results while streamlining everyday sample preparation workflows.

This poster explores automated and semi-automated workflows that leverage positive pressure processing for small-volume sample preparation using micro-elution plates. The study evaluates how positive pressure technology can improve flow consistency, maximize analyte recovery from limited sample volumes and deliver the reproducible performance needed for accurate and reliable analytical results.

Efficiently desalt diverse peptides with Tecan Narrow Bore Extraction™ (NBE) columns. Reduce drying time, automate SPE cleanup, and prepare high quality samples for LC MS/MS.

Streamline proteomics with Tecan’s NBE format for rapid on-column digestion, minimizing sample loss and boosting analyte recovery.

This note demonstrates efficient PFAS extraction from contaminated surface water using automated mixed-mode µSPEed® cartridges and Tecan's Resolvex® Prep.

Tecan’s proprietary NBE™ format enables digestion or incubation AND elution in a single SPE column. Read this app note to learn more and streamline your workflows!

Tecan’s proprietary NBE format SPE columns enable desalting in the column before peptide extraction. Read this app note to see how Tecan can accelerate your workflows!