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© 2022 MJH Life Sciences and Chromatography Online. All rights reserved.
© 2022 MJH Life Sciences™ and Chromatography Online. All rights reserved.
Vijesh Kumar is a graduate student at the Department of Chemical Engineering, Indian Institute of Technology, New Delhi, India.
In this article, we discuss the role of the gradient in protein separations by reversed-phase and ionexchange high performance liquid chromatography (HPLC).
A discussion of the role of the gradient in separation by reversed-phase and ion-exchange HPLC, with data from two products to illustrate the key points
The use of a monolithic column reduces analysis time in an on-line biopharmaceutical HPLC analysis of granulocyte colony stimulating factor.