YMC Europe GmbH

The recently introduced 1 mm YMC-Triart C18 UHPLC column was used for the high sensitivity LC–MS screening of six antineoplastic drugs.

A YMC-Triart C18 and a YMC-Triart SIL column were used in RP and NP mode, respectively, for the analysis of four different phosphoramidites.

Mipomersen was analyzed by LC–HRMS using a biocompatible YMC-Triart C8 metal-free column, ensuring symmetric peaks, high sensitivity, and no carryover effects.

The separation of three cell-penetrating peptides (CPPs) was performed using a YMC-Triart C18 UHPLC column, providing sharp peaks for a fast analysis in under 4 min.

Twenty-eight PFAS in human serum were analyzed using a YMC-Triart C18 column. An automated SPE system was coupled to an online SPE column-switching LC–MS/MS method.

Four hydrophilic dipeptides were separated with excellent resolution using a YMC-Triart SIL column. Even structurally similar dipeptides were separated in under 4 min.

The UHPLC analysis of four cyclic peptides is shown. Based on the hydrophobicity of the peptides, a YMC-Triart Bio C4 UHPLC column was chosen for this application.

The analysis of FMOC-derivatized glyphosate and AMPA in water samples was performed using a YMC-Triart C18 UHPLC column at an elevated pH of 9.5.

The analysis of FMOC-derivatized (fluorenmethyloxycarbonyl) glyphosate and AMPA according to DIN ISO 16308 is performed using a YMC-Triart C18 UHPLC column at pH 9.5.

Six nitrosamines are separated using a YMC-Triart C18 UHPLC column. This is challenging due to the commonly observed small peaks thanks to low impurity concentrations.

Six nitrosamines are separated using a YMC-Triart C18 UHPLC column. This is challenging due to the commonly observed small peaks thanks to low impurity concentrations.

A fast separation of remdesivir and its active metabolite GS-441524 for the treatment of COVID-19 is obtained in only 3 minutes using a YMC-Triart C18 UHPLC column.

Charge variants of 28 mAbs were analyzed under MS-compatible conditions using YMC’s BioPro IEX SF column. Each mAb of different pI was separated from its variants

Charge variants of 28 mAbs were analyzed under MS-compatible conditions using YMC’s BioPro IEX SF column. Each mAb of different pI was separated from its variants

In reference to the Japanese Pharmacopeia, YMC-Triart Bio C18 was used to analyze the peptide leuprorelin (GnRH analogue) and separate it from its degradation products.

In this application note, YMC’s dedicated SEC column for antibodies, YMC-SEC MAB, has been used to develop a method for bevacizumab using MALLS detection.

Charge variants of 28 mAbs were analyzed under MS-compatible conditions using YMC’s BioPro IEX SF column. Each mAb of different pI was separated from its variants

Mipomersen was analyzed by LC–HRMS using a biocompatible YMC-Triart C8 metal-free column ensuring symmetric peaks, high sensitivity, and no carryover effects.

The antibody-drug conjugate was separated from its aggregates and fragments in only one run using a YMC-SEC MAB column and varying mobile phases.

In this application note, the DAR of the SigmaMAb ADC-mimic was determined using YMC’s BioPro HIC BF column and a nanospray ionization mass spectrometer.

Mipomersen (Kynamro®) is used as a model for the analysis of antisense oligonucleotides (ASOs) using LC-HRMS. The YMC-Triart C8 metal-free column used is an excellent choice for oligonucleotides.

The baseline separation of various chlorophenols, that only differ in the amount and/or position of the chlorine substituents, is made possible using YMC-Triart C18 ExRS.

YMC’s latest HIC column is used to analyse the DAR of Brentuximab Vedotin allowing higher flow rates and shorter run times thanks to the pressure resistant particle.

YMC’s HIC columns, BioPro HIC HT, and BioPro HIC BF address separation requirements of different compounds. The separation behaviour of two mAbs is demonstrated.

This application note shows how a mixture of seven in-house mAbs, as well as the molecular variants of two mAbs, were characterised via HIC-MS using YMC’s BioPro HIC BF column.

In this application note the DAR of the SigmaMAb ADC-mimic was determined using YMC’s BioPro HIC BF column and a nanospray ionisation mass spectrometer.

Monoclonal antibodies and their oxidised species are separated using YMC’s more hydrophobic BioPro HIC BF column, dedicated for low hydrophobicity proteins and oxidised mAb variants.

Four therapeutic mAbs of different species, isotypes, and pI together with their variants were analysed using pH and salt gradient mode using YMC’s non-porous BioPro IEX SF.

YMC-Triart Bio C4 is used in YMC capillary column format to analyse and identify intact commercial mAbs by micro LC–MS/MS at an elevated temperature of 75°C.