Steroids represent a chemically distinct class of hormones with wide-ranging biological functions. Synthetic derivatives of endogenous steroid prototypes are used medically in birth control and in the treatment of asthma, arthritis, inflammation, and osteoporosis.
This application note demonstrates how comprehensive 2D-LC can be used to resolve the complex mixture of hydrophilic phenols found in virgin olive oil and investigates differences in the phenol composition of several olive oils.
Selectivity is the most powerful tool to optimize separations in HPLC. This parameter is changed by using different bonded phases, including C18, polar embedded, phenyl bonded phases and perflorophenyl, or by changing the mobile phase. In this work, 4.6 ? 50 mm Poroshell 120 columns are used to quickly evaluate method development choices for the analysis of non-steroidal anti-inflammatory drugs (NSAIDS).
A new fused-core particle designed specifically for the separation of proteins and monoclonal antibodies with 3.4 ?m particle size and a thin 0.2 ?m porous shell demonstrates high resolution of the heavy and light chain fragments of IgG1.