The Application Notebook-06-01-2014
The Application Notebook
Separation of a Mix of Acidic, Basic, and Neutral Compounds at High pH Conditions
June 01, 2014
General
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The unique surface chemistry of the Flare diamond core-shell column combines ionic and hydrophobic separation mechanisms to effectively retain a variety of chemical species in a single run.
Structural Differences in Modified Starches
June 01, 2014
Food & Beverage
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Modified starches are important materials used in many applications including foodstuffs.
Characterization of Tobacco Extracts by Gas Chromatography High Resolution Time of Flight Mass Spectrometry (GC-HRT)
June 01, 2014
Medical/Biological
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High resolution time-of-flight mass spectrometry was used for comprehensive profiling of tobacco plant extracts.
Characterization of PLGA Using SEC–MALS-VIS
June 01, 2014
Pharmaceutical/Drug Discovery
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Poly(lactic-co-glycolic acid) (PLGA) is a copolymer based on glycolic acid and lactic acid. The two monomer units are linked together by ester linkages and form linear polyester chains.
Separation of Five Steroids on a C18-Functionalized Polymeric Reversed-Phase HPLC Column (PRP-C18)
June 01, 2014
Pharmaceutical/Drug Discovery
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Steroids represent a chemically distinct class of hormones with wide-ranging biological functions. Synthetic derivatives of endogenous steroid prototypes are used medically in birth control and in the treatment of asthma, arthritis, inflammation, and osteoporosis.
The Analysis of Chlorinated Dioxins, Difurans, and Polychlorinated Biphenyls in Edible Oils
June 01, 2014
Food & Beverage
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The dioxin family consists of 210 compounds, of which 17 contain the 2,3,7,8 pattern of chlorination.
Concentrating Phenolic Compounds Using the XcelVap Concentration System
June 01, 2014
General
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Natural and synthetic phenolic compounds have been used in a wide range of applications, from antiseptics and fungicides to food additives.
Keeping Water Safe: Detecting Pharmaceutical and Personal Care Products in Water Using Liquid Chromatography–Mass Spectrometry
June 01, 2014
Environmental
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Pharmaceutical and personal care products (PPCPs) are products used for personal health or cosmetic reasons.
Recent Improvements in Time of Flight Mass Spectrometer Detection Technologies
June 01, 2014
General
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In mass spectrometry instrumentation, the sensitivity and resolution of the instrument is often limited by the detector.
Utilization of CESI Technology for Comprehensive Characterization of Biologics
June 01, 2014
Medical/Biological
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Monoclonal antibodies (mAbs) form a major class of biologics and recently biosimilars and biobetters are being added to the growing inventory of therapeutics.
Intrada Amino Acid Separates Intact Amino Acids in Human Serum
June 01, 2014
Medical/Biological
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We have previously shown the ability of a novel stationary phase to separate 55 amino acids without derivatization (LCGC 32(3), 211 [2014]).
Measuring Antibody Molecular Weight by SEC-MALS
June 01, 2014
Pharmaceutical/Drug Discovery
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A purified polyclonal antibody (IgG) is separated and fully characterized using the Viscotek SEC-MALS 20, allowing calculation of molecular weight and radius of gyration (Rg).
Isolation of Structurally Related Components in Natural Product Extracts Using the RegisPack and Whelk-O1 Chiral Selectors
June 01, 2014
Chiral
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Chiral separations of multi-component natural product mixtures comprised of enantiomers, diastereomers, and unrelated molecules are often difficult to resolve in a single isocratic method.
Solid Phase Extraction of Organophosphorus Pesticides and Triazine Herbicides in Water Using a New Polymeric Sorbent
June 01, 2014
Environmental
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This application describes a solid phase extraction (SPE) method for EPA method 8141B analytes, including organophosphorus compounds, and triazine herbicides in water.
Analysis of Alprostadil by HPLC with Post-Column Derivatization
June 01, 2014
Pharmaceutical/Drug Discovery
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Alprostadil (Prostaglandin E1) is a drug that has vasodilation properties and is used to treat erectile dysfunction and other medical conditions.
Separation of Nucleobases Using TSKgel SuperSW mAb HTP Column in HILIC Mode
June 01, 2014
Medical/Biological
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Hydrophilic interaction liquid chromatography (HILIC) is one of the fastest growing modes of separation, in which any polar chromatographic surface can be used.
Detection of Low-Level Sulfur Compounds in Spearmint Oil Using the Pulsed Flame Photometric Detector (PFPD)
June 01, 2014
Food & Beverage
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Two species of spearmint oil (mentha spicata and mentha gracilis) are cultivated in the United States.
Accurate Pain Management Analysis in Under 5 Min on Raptor Biphenyl Superficially Porous Particle LC Columns
June 01, 2014
Pharmaceutical/Drug Discovery
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Pain management LC analyses can be difficult to optimize due to the limited selectivity of C18 and phenyl-hexyl phases.
HILIC with Increased Sensitivity for the Analysis of Sugars
June 01, 2014
Food & Beverage
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Hydrophilic interaction chromatography (HILIC) provides an alternative approach for the separation of highly polar substances.
Antibody Drug Conjugate (ADC) Analysis
June 01, 2014
Pharmaceutical/Drug Discovery
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A novel method for drug antibody ratio determinations based on SEC-MALS in conjunction with UV absorption and differential refractive index detection.
Veterinary Drug Residue Analysis Using the AutoMate-Q40: An Automated Solution to QuEChERS
June 01, 2014
Food & Beverage
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QuEChERS is a quick-easy-cheap-effective-rugged-safe extraction method developed in 2003 for the extraction of pesticide residues in agricultural commodities (1,2).
Determination of Phenolic Compounds in Virgin Olive Oil Using Comprehensive 2D-LC
June 01, 2014
Food & Beverage
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This application note demonstrates how comprehensive 2D-LC can be used to resolve the complex mixture of hydrophilic phenols found in virgin olive oil and investigates differences in the phenol composition of several olive oils.
Fast Screening Methods for Analgesics and Non-Steroidal Anti-Inflammatory (NSAIDS) Drugs by HPLC with Agilent Poroshell 120 Columns
June 01, 2014
Pharmaceutical/Drug Discovery
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Selectivity is the most powerful tool to optimize separations in HPLC. This parameter is changed by using different bonded phases, including C18, polar embedded, phenyl bonded phases and perflorophenyl, or by changing the mobile phase. In this work, 4.6 ? 50 mm Poroshell 120 columns are used to quickly evaluate method development choices for the analysis of non-steroidal anti-inflammatory drugs (NSAIDS).
High Resolution LC–MS Analysis of Reduced IgG1 Monoclonal Antibody Fragments Using HALO Protein C4
June 01, 2014
Medical/Biological
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A new fused-core particle designed specifically for the separation of proteins and monoclonal antibodies with 3.4 ?m particle size and a thin 0.2 ?m porous shell demonstrates high resolution of the heavy and light chain fragments of IgG1.
Vol 32 No 6 LCGC North America June 2014 Application Notebook PDF
June 01, 2014
Issue PDF
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Click the title above to open the LCGC North America June 2014 Application Notebook, Vol 32 No s6, in an interactive PDF format.