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The Application Notebook-06-01-2014

The Application Notebook

Separation of Five Steroids on a C18-Functionalized Polymeric Reversed-Phase HPLC Column (PRP-C18)

June 01, 2014

Pharmaceutical/Drug Discovery

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Steroids represent a chemically distinct class of hormones with wide-ranging biological functions. Synthetic derivatives of endogenous steroid prototypes are used medically in birth control and in the treatment of asthma, arthritis, inflammation, and osteoporosis.

Determination of Phenolic Compounds in Virgin Olive Oil Using Comprehensive 2D-LC

June 01, 2014

Food & Beverage

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This application note demonstrates how comprehensive 2D-LC can be used to resolve the complex mixture of hydrophilic phenols found in virgin olive oil and investigates differences in the phenol composition of several olive oils.

Fast Screening Methods for Analgesics and Non-Steroidal Anti-Inflammatory (NSAIDS) Drugs by HPLC with Agilent Poroshell 120 Columns

June 01, 2014

Pharmaceutical/Drug Discovery

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Selectivity is the most powerful tool to optimize separations in HPLC. This parameter is changed by using different bonded phases, including C18, polar embedded, phenyl bonded phases and perflorophenyl, or by changing the mobile phase. In this work, 4.6 ? 50 mm Poroshell 120 columns are used to quickly evaluate method development choices for the analysis of non-steroidal anti-inflammatory drugs (NSAIDS).

High Resolution LC–MS Analysis of Reduced IgG1 Monoclonal Antibody Fragments Using HALO Protein C4

June 01, 2014

Medical/Biological

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A new fused-core particle designed specifically for the separation of proteins and monoclonal antibodies with 3.4 ?m particle size and a thin 0.2 ?m porous shell demonstrates high resolution of the heavy and light chain fragments of IgG1.

Vol 32 No 6 LCGC North America June 2014 Application Notebook PDF

June 01, 2014

Issue PDF

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Click the title above to open the LCGC North America June 2014 Application Notebook, Vol 32 No s6, in an interactive PDF format.