Separation of a Mix of Acidic, Basic, and Neutral Compounds at High pH Conditions

June 1, 2014

The Application Notebook

The Application Notebook, The Application Notebook-06-01-2014, Volume 0, Issue 0

The unique surface chemistry of the Flare diamond core-shell column combines ionic and hydrophobic separation mechanisms to effectively retain a variety of chemical species in a single run.

The unique surface chemistry of the Flare diamond core-shell column combines ionic and hydrophobic separation mechanisms to effectively retain a variety of chemical species in a single run.

HPLC Conditions

Column Name: Flare C18 Mixed-Mode Column

Dimensions: 4.6 × 33 mm, 3.6 µm, 180 Å

HPLC System: Waters® 1525 Binary Pump

Injection Volume: 5 µL

Detection: UV at 254 nm

Flow Rate: 1.0 mL/min

Separation Mode: Isocratic

Mobile Phase: A: 10 mM phosphate buffer, pH 12; B: acetonitrile A/B (70:30)

Temperature: 35 °C

Analytes:

Conclusions

1) Fast separation at high pH

2) Separation possible with 100% aqueous mobile phase

3) Straightforward method transfer to any system

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