Separation of a Mix of Acidic, Basic, and Neutral Compounds at High pH Conditions
The unique surface chemistry of the Flare diamond core-shell column combines ionic and hydrophobic separation mechanisms to effectively retain a variety of chemical species in a single run.
The unique surface chemistry of the Flare diamond core-shell column combines ionic and hydrophobic separation mechanisms to effectively retain a variety of chemical species in a single run.
HPLC Conditions
Column Name: Flare C18 Mixed-Mode Column
Dimensions: 4.6 × 33 mm, 3.6 µm, 180 Å
HPLC System: Waters® 1525 Binary Pump
Injection Volume: 5 µL
Detection: UV at 254 nm
Flow Rate: 1.0 mL/min
Separation Mode: Isocratic
Mobile Phase: A: 10 mM phosphate buffer, pH 12; B: acetonitrile A/B (70:30)
Temperature: 35 °C
Analytes:
Conclusions
1) Fast separation at high pH
2) Separation possible with 100% aqueous mobile phase
3) Straightforward method transfer to any system
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