The Column-01-19-2016

The Column

The Power of 2D LC

January 19, 2016

Cover Story

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Protein biopharmaceuticals have seen an enormous growth in the last decade, and as a result, separation scientists are giving increased attention to methods for characterizing biopharmaceuticals. One powerful technique for analyzing proteins is two-dimensional liquid chromatography (2D LC). Gerd Vanhoenacker of the Research Institute for Chromatography (RIC) in Kortrijk, Belgium, has been conducting research into peptide mapping of therapeutic monoclonal antibodies (mAbs) using 2D LC. He recently spoke to The Column about this work.

Pattern Modulation Offers Alternative to Pulse Modulation in GC×GC

January 19, 2016

News

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New research into flow modulation methods in valve-based two-dimensional gas chromatography (GC×GC) has produced an effective alternative to traditional pulse modulation.1 Described as “pattern modulation”, this new method increases effluent to the secondary column with flow rates compatible with most chromatographs and spectrometers.

Eastern Analytical Symposium Summary

January 19, 2016

News

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Summary of the Eastern Analytical Symposium 2015.

Metabolomic Detection of Early-Stage Ovarian Cancer

January 19, 2016

News

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A detection method for early-stage ovarian cancer has been developed using ultrahigh‑pressure liquid chromatography–high-resolution mass spectrometry (UHPLC–HRMS) in combination with tandem MS–MS.

Extending the Detection Limits for the Analysis of Organotin Contaminants Using Soft Ionization

January 19, 2016

Features

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The inherent sensitivity and selectivity of time-of-flight mass spectrometry (TOF-MS) can be augmented by soft electron ionization (EI) to provide ultratrace-level quantitation of organotins in complex environmental extracts. These organotin species are a focus of current concern as environmental contaminants, but analysis using conventional 70 eV ionization energies is made difficult by their propensity to undergo extensive fragmentation. The use of soft EI helps to solve this problem by producing simplified spectra with enhanced diagnostic ions.

The LCGC Blog: Column Overload in Gas Chromatography with Vacuum Ultraviolet Detection

January 19, 2016

Features

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Column overload is a very commonly encountered issue in gas chromatography (GC) for beginners. Changes in peak symmetry, generally observed as peak fronting, can be subtle in the sharp peaks generated by GC, but the result can be significant shifts in retention times, loss of resolution, and error in peak integration. LCGC Blogger Kevin Schug explains more.

Vol 12 No 1 The Column January 19, 2016 Europe and Asia PDF

January 19, 2016

Issue PDF

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Click the title above to open The Column January 19, 2016 Europe & Asia issue, Volume 12, Number 1, in an interactive PDF format.

Vol 12 No 1 The Column January 19, 2016 North American PDF

January 19, 2016

Issue PDF

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Click the title above to open The Column January 19, 2016 North American issue, Volume 12, Number 1, in an interactive PDF format.