The Column-09-21-2018

The Column

Waters and Restek Announce Co-Marketing Agreement

September 21, 2018

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Waters and Restek have entered into a co-marketing agreement aimed at food safety laboratories and promoting the use of Waters gas chromatography–mass spectrometry instruments with Restek GC consumables.

Retroactive Toxicology Study on Adolescents Using GC–MS

September 21, 2018

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Toxicological surveillance of illicit prescription and illegal drug abuse has been carried out using post-mortem data from Clark Country, Nevada, USA, with GC–MS, headspace GC–MS, GC×GC–MS, LC–MS, and ELISA.

Shimadzu Receive NRW.Invest Award

September 21, 2018

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Shimadzu has been awarded the 2018 NRW.Invest Award by the North Rhine‑Westphalia (NRW) region of Germany.

Combining Sorptive Extraction with Two-Dimensional Gas Chromatography for the Flavour Profiling of Milk

September 21, 2018

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This proof-of-principle study shows that polymer-based sorptive extraction probes, coupled with secondary focusing by thermal desorption and analysis by flow-modulated GC×GC–TOF-MS/FID, can be used to separate and identify flavour compounds in milk. As well as comparing the profiles of dairy and non-dairy milks, this article highlights the practical benefits of this sampling procedure, the ability of two-dimensional GC to physically separate components that would coelute in one-dimensional GC, and the use of software tools to improve workflow.

Best Practices for Analyzing Pesticides and Their Metabolites in Environmental Samples

September 21, 2018

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When a company wishes to commercialize a new pesticide, they must conduct environmental studies and develop analytical methods capable of detecting the pesticide, and its metabolites, in soil and water samples. The methods must be robust and rugged, for easy use in routine analysis. James Stry, a principal investigator at FMC Agricultural Solutions, recently talked to LCGC about best practices he and his team have established for developing such methods, including approaches to meeting a variety of requirements of regulatory bodies, simplifying sample preparation, dealing with matrix effects, choosing an ionization method, and streamlining method development.

Enhancing PLGA Characterization with Multi-Angle Light Scattering and Differential Viscometry

September 21, 2018

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The functional properties of polymers, such as poly(lactic-co-glycolic acid) (PLGA), relevant to drug delivery and biomedical devices, are governed by the molecular properties of molar mass, composition, conformation, and branching. This article demonstrates how such polymers are fully characterized, quickly and absolutely, using gel permeation chromatography (GPC) with multi-angle light scattering (MALS) and online viscometry.

Automated Multicolumn Purification of a Histidine-Tagged Protein

September 21, 2018

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Approximately 40% of recombinant proteins that are purified use a histidine tag for easy capture. This article covers how to automate the purification of histidine-tagged proteins and how purification conditions can be optimized to an automated four-step purification scheme that uses affinity-, ion exchange-, and size-exclusion columns. Using a multistep purification scheme removes the manual steps that cause loss of precious proteins and take more time, like dialysis, collection, and reinjecting samples. The final purification scheme reduces a 3–4-day process to 11.5 h from start to finish, all while improving reproducibility, yield, and comparable purity.

Vol 14 No 9 The Column September 2018 North American PDF

September 21, 2018

Issue PDF

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Click the title above to open The Column September 2018 North American issue, Volume 14, Number 9, in an interactive PDF format.

Vol 14 No 9 The Column September 2018 Europe and Asia PDF

September 21, 2018

Issue PDF

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Click the title above to open The Column September 2018 Europe & Asia issue, Volume 14, Number 9, in an interactive PDF format.