LCGC Asia Pacific-11-01-2019

Click the title above to open the LCGC Asia Pacific November/December 2019 regular issue, Vol 22, No 4, in an interactive PDF format.

The versatile size-exclusion ultrahigh‑performance liquid chromatography (SE-UHPLC) platform method described here provides superior separation for bispecific monoclonal antibody formats compared to a previous method.

The sample solvent can have a big impact on peak shape in both reversed-phase and hydrophilic interaction liquid chromatography (HILIC) separations, especially when large volumes are injected. Diluting the sample with weak solvent can be an effective solution to mitigate this problem, but we have to be careful to not lose analytes of interest to precipitation or phase separation.

Most manufacturers ship gas chromatographs with a small collection of consumable parts and accessories, such as extra ferrules, inlet liners, or septa, and a few instrument-specific tools. In earlier times, some convenience items might have been included as well, like a bubble flow meter or a small set of tools, but now it’s rare to find such things in the shipping boxes. This edition of “GC Connections” lists a number of essential items that should be on hand in every gas chromatography (GC) laboratory, their function, and how to use them effectively.

An excerpt from LCGC’s professional development platform, CHROMacademy.com