LCGC Europe-07-01-2013

A new method to optimize liquid chromatography (LC) methods using a Quality by Design (QbD) approach is presented. This method is based on the use of design of experiments (DOE) and independent component analysis (ICA) to accurately estimate the modeled responses (that is, the retention times at the beginning, the apex, and the end) of each peak, even for coeluted peaks. This method was applied to the optimization of the separation of nine compounds in a mixture, yielding the design space and the demonstration of robustness of the method.

Events

The underlying principles of gradient elution HPLC are very different from those of the isocratic mode. Appreciating the differences can lead to better methods.

The concluding part of the article from last month's "Question of Quality" on what "complete data" actually means in practice.

This article discusses the role of CE for vitamin analysis and highlights a number of practical applications.

The reasons that make HPLC so ubiquitous; the fundamentals on how we conduct HPLC separations; and a few opportunities with far-reaching impacts in life sciences for separation scientists are discussed in this article.

Can anything be done to correct for baseline drift in gradient separations?

In this instalment, John Hinshaw compares the GC separation process to a long-distance run through a long corridor with some unique properties.

Click the title above to open the LCGC Europe July 2013 regular issue, Vol 26 No 7, in an interactive PDF format.