This article describes possible sources of error that may contribute to significant inaccuracy in peptide assay methods: The fi ltration material (cellulose) used in sample clean up and the vial material (glass and plastic) used in LC analysis. This study is based on ?-endorphin but has relevance for most peptide assays.
HPLC method development is an arduous process requiring considerable experience and scientific judgement. In this article, a roadmap for rapid HPLC method development using a three-pronged approach is presented.
A practical and reliable analytical procedure that incorporates MDGC with a planar microfl uidic device as a Deans switch was developed for the characterization of chlorinated degreasers in marine gas oil.