LCGC Europe-10-01-2018

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Glycosylation is a critical quality attribute (CQA) that can impact on product safety and efficacy of protein biopharmaceuticals. Characterization of N-glycans is therefore of paramount importance for the pharmaceutical industry. Hydrophilic interaction liquid chromatography (HILIC) combined with fluorescence detection (FLD) and 2-aminobenzamide (2-AB) labelling is the golden standard for the analysis of N-glycans enzymatically liberated from biopharmaceuticals. However, for phosphorylated N-glycans, that is, those attached on lysosomal enzymes, irreproducible data and recovery issues are observed on conventional liquid chromatography (LC) instrumentation and columns, which can be attributed to the interaction of the phosphate moieties with stainless steel components in the flow path. This article demonstrates the analysis of phosphorylated glycans with full recovery on a bio-inert LC system and PEEK-lined HILIC column.

This instalment provides an overview of the modern trends and best practices in mobile-phase selection for reversed-phase liquid chromatography (LC). In particular, we focus on selection criteria and rationales for enhancing analytical performance and ease of preparation.

The use of ultrahigh-pressure liquid chromatography (UHPLC) is now commonplace among pharmaceutical laboratories. However, until depreciation cycles replace traditional high performance liquid chromatography (HPLC) systems that operate at a maximum pressure of 400 bar, the advantages of UHPLC cannot be realized worldwide. Thus, product methods developed using UHPLC capabilities cannot directly transfer these methods to receiving laboratories without qualified UHPLC availability.

What types of mixers are needed with different types of liquid chromatography (LC) pumps? Are different mixers needed for different applications?

LCGC Europe spoke to Guilherme L. Alexandrino from the University of Copenhagen, Denmark, about the benefits of combining a modern “pixel-based” chemometrics technique with comprehensive two‑dimensional gas chromatography (GC×GC) in petroleomics applications.

The 9th SCM symposia (SCM-9) will take place from 30 January to 1 February 2019, in Amsterdam, The Netherlands.