LCGC Europe-12-01-2015

In his final “Column Watch” instalment, Ron Majors looks into his crystal ball and discusses future needs in the areas of high performance liquid chromatography (HPLC) and ultrahigh-pressure liquid chromatography (UHPLC) column technology and related instrumentation.

Particulate matter from the sample or the system can cause havoc in the chromatogram.

Click the title above to open the LCGC Europe December 2015 regular issue, Vol 28, No 12, in an interactive PDF format.

Characterization of macromolecules and colloids is an area of considerable interest. Asymmetrical flow field-flow fractionation (AF4) has become a well-established method, but many potential users possess limited knowledge of its capabilities, or how it can provide additional information or serve as validation to the traditional analytical techniques. This review article highlights several practical applications where AF4 should be given special consideration, and discusses benefits and drawbacks of the different methods.

Characterization of macromolecules and colloids is an area of considerable interest. Asymmetrical flow field-flow fractionation (AF4) has become a well-established method, but many potential users possess limited knowledge of its capabilities, and that it can provide additional information or serve as validation to the traditional analytical techniques. This review article highlights several practical applications in which AF4 should be given special consideration, and discusses benefits and drawbacks of the different methods.