January/February 2025

Christopher A. Pohl and Katelynn A. Perrault Uptmor are the winners of the 18th annual LCGC Lifetime Achievement and Emerging Leader in Chromatography Awards, respectively. The LCGC Awards honor the work of talented separation scientists at different stages in their career (See Table I, accessible through the QR code at the end of the article). The award winners will be honored during an oral symposium at the Pittcon 2025 conference held March 1-5, in Boston, Massachusetts.

In Part II of our exploration of splitless injection, we will see that it is a surprisingly complex process, and that it is difficult to understand because we cannot see what is happening during the injection process. For this discussion, we will think of the injection process as beginning with the syringe plunger being depressed and ending with the start of a temperature program in the column oven. In most splitless injections, this process requires 30 s to 1 min. There are several band broadening and focusing mechanisms that affect the peak shapes, widths, and heights resulting from splitless injection.

This article delves into advancements in green chromatography, focusing on how innovative HPLC column design can drive eco-conscious practices.

In this installment, I primarily discuss considerations to keep in mind when choosing buffering additives that will be used for LC methods involving UV absorbance detection.

Click here to access the LCGC International January/February 2025 Europe PDF in an interactive format.