LCGC North America-06-01-2015

This installment provides an overview of modern practices of high-throughput purification to support small-molecule drug discovery.

The use of superficially porous particles in the manufacture of high performance liquid chromatography (HPLC) columns has become prominent in recent years. Over the course of the past decade most major manufacturers have built column lines around the technology.

Readers' questions regarding problems related to internal standard calibration of liquid chromatography methods are addressed.

The HPLC Troubleshooting app is designed to help beginner HPLC users with HPLC troubleshooting.

This work presents an on-line combination of a simple microgradient device for reversed-phase liquid chromatography (LC) and electrospray ionization (ESI) tandem mass spectrometry (MS-MS).

We have developed a miniaturized, multifunctional device, called the "Single-Probe," that is capable of probing small targets and of sampling and ionizing molecular species.

Temperature affects not only retention but also relative retention in gas chromatography (GC) and therefore, when we change temperature, we also change the selectivity of the separation. This is true as we alter the isothermal separation temperature, but also as we change the slope of the temperature program gradient.

Caroline West, the winner of the 2015 LCGC Emerging Leader in Chromatography award, has a diverse set of scientific interests-in high performance liquid chromatography (HPLC), supercritical fluid chromatography (SFC), hydrophilic-interaction chromatography (HILIC), and enantioselective separations-but all are primarily focused on the fundamentals of chromatographic selectivity.

Click the title above to open the LCGC North America June 2015 regular issue, Vol 33 No 6, in an interactive PDF format.