Adapting Well Known Chromatographic Techniques for the Analysis of Biomolecules

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Learn how to optimize your biomolecule methods and when to use ion pair reagents to improve your protein, peptide and glycan separations Live Tuesday, Sep. 29, 2020 at 1pm EDT | 12pm CDT | 10am PDT On demand available after airing until Sep. 29, 2021 Register free: Register free

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Event Overview:
Regulatory guidelines (ICH Q6B) state that a detailed characterization of all therapeutic biomolecules must be carried out prior to any new product launch. It is important to confirm primary structure and identify any post translational modifications (PTMs) that have occurred during manufacturing processes. When considering biosimilars, a comparison should be made with the originator therapeutic. Chromatography is a well-established technique for the analysis of small-molecule therapeutics and their related substances and stability and is useful for both quantitation and quality purposes. In this webcast we discuss how you can draw from experience with small-molecule method development to optimize biomolecule methodologies. We also discuss how ion-pair reagents can be added to the mobile phase to enhance the interaction of more polar biomolecules like oligonucleotides, and lead to excellent separations even to the n+/-1 level. Whether you are working with peptides or proteins, this webcast aims to provide ideas on how to approach their analysis using well understood and recognized modes of chromatography

Key Learning Objectives

  • Learn how to optimize biomolecule methodologies

  • Learn when to consider ion pair reagents for polar biomolecules

  • Run through examples of various biomolecule separations and methods

Speaker: Gemma Lo, Senior Product Manager, Avantor

Time and Date: Tuesday, Sept. 29, 2020 at 1pm EDT | 12pm CDT | 10am PDT

On demand available after airing until Sept. 29, 2021

Sponsor: Mac Mod Analytical

Register free: