This study investigates the separation of egg white proteins using flash and prep HPLC with silica stationary phases varying in pore and particle sizes. Larger pore sizes improved protein interaction with the stationary phase, while particles above 10 µm were ineffective. Optimal separation was achieved using the Pure Excellence C-950 with a PrepPure C18WP column, combining wide pores, small particles, and a step gradient.
In this fifth part of " Conversations with Michael Dong," a series on fundamentals of HPLC in pharmaceutical analysis, LCGC International’s "Perspectives in HPLC" column editor discusses how to overcome challenges with reversed-phase chromatography in drug analysis.
This week on Chromatography Online, leading experts shared practical insights with LCGC International on sustainable affinity methods, siRNA impurity profiling, nontarget screening, peptide tech transfer, and AI in separation science.
Anneli Kruve reports that toxicity predictions from MS2-derived molecular fingerprints outperform spectral-similarity methods in nontarget screening of food.
In this fourth part of " Conversations with Michael Dong," a series on fundamentals of HPLC in pharmaceutical analysis, LCGC International’s "Perspectives in HPLC" column editor explains why buffers are most often used in Mobile Phase A in high-pressure liquid chromatography of pharmaceuticals.
