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Liquid chromatography (LC) separations performed at elevated temperatures offer the following advantages.
A reader recently asked:
What are some of the advantages of LC separations at high temperatures?
Liquid chromatography (LC) separations performed at elevated temperatures offer the following advantages:
Limitations on the use of high temperatures for LC separations include the thermal stability of the stationary phase and analytes, although the analytes are exposed to the higher temperatures for a relatively short time.
LCGC's "LC Troubleshooting" columnist John Dolan identified other possible limitations: "High temperature operation requires an oven that can support the desired temperature and a mechanism to preheat the mobile phase to the column temperature. Most commercial systems cannot heat above 60-70 C, and performance at these temperatures varies widely with the product. Temperature mismatch of the mobile phase and column causes a thermal gradient along the column, resulting in broadened or split peaks."
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