Automated DNA Fragment Analysis by High Performance Ultrathin-Layer Agarose Gel Electrophoresis

Published on: 
LCGC North America, November 1999, Volume 17, Issue 11
Pages: 1020–

Ultrathin-layer agarose gel electrophoresis combines the advantages of the multilane format of conventional slab and the performance of capillary gel electrophoresis. This article describes the detection of separated sample components in real-time by using an integrated scanning laster-induced fluorescence–avalanche photodiode detection system. Ultrathin-layer agarose gel electrophoresis is a novel and high performance separation technique that provides a simple platform for fast deoxyribonucleic acid fragment analysis with the advantage that the same gel-filled cassette can be used simultaneously for a size range of 20–23,130 base pairs. The author provides examples of rapid genotyping and high-throughput mutation analysis of polymerase chain reaction-generated fragments.