Automated DNA Fragment Analysis by High Performance Ultrathin-Layer Agarose Gel Electrophoresis
Ultrathin-layer agarose gel electrophoresis combines the advantages of the multilane format of conventional slab and the performance of capillary gel electrophoresis. This article describes the detection of separated sample components in real-time by using an integrated scanning laster-induced fluorescence–avalanche photodiode detection system. Ultrathin-layer agarose gel electrophoresis is a novel and high performance separation technique that provides a simple platform for fast deoxyribonucleic acid fragment analysis with the advantage that the same gel-filled cassette can be used simultaneously for a size range of 20–23,130 base pairs. The author provides examples of rapid genotyping and high-throughput mutation analysis of polymerase chain reaction-generated fragments.
LCGC 17(11), 1020–1026 (1999).
Microseparations and Analysis Covered at HPLC 2024
July 23rd 2024At HPLC 2024 in Denver, Colorado, various sessions will be led by experienced scientists, covering different aspects of chromatography techniques and maximizing their effectiveness. Here is some information about tomorrow’s sessions.
Characterization of Product Related Variants in Therapeutic Monoclonal Antibodies
July 16th 2024Navin Rauniyar and Xuemei Han of Tanvex Biopharma USA recently discussed how identifying product-related variants through characterization enables the recognition of impurities that compromise the quality and safety of drugs.
