Monoliths as Stationary Phases for Separating Biopolymers – Fourth-Generation Chromatography Sorbents

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LCGC North America, November 1999, Volume 17, Issue 11
Pages: 998–1005

Columns | <b>Column: Column Watch</b>

Monoliths are chromatography sorbents cast as a homogenous phase into chromatography columns as a single, continuous piece. In contrast, regular chromatographic solvents are packed as individual particles. In this month's "Column Watch," the guest columnists compare three of these novel sorbents with a conventional packed-particle column in terms of porosity, static, and dynamic capacity. Monoliths show flow rate–independent separation efficiency and dynamic capacity as well as higher porosity than conventional columns.