Because of toxicity concerns and a lack of relevant case studies, displacement chromatography has yet to be embraced by the biotechnology industry. This article is an example of a protein displacement process used at the pilot scale.
LCGC 17(11), 1028–1035 (1999).Multi-Step Preparative LC–MS Workflow for Peptide Purification
March 21st 2025This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.