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|Articles|November 1, 1999

LCGC North America

  • November 1999
  • Volume 17
  • Issue 11
  • Pages: 1010–1015

A Coin-Toss Experiment, Part II – Limits of the Analogy

Concluding a two-part column series, this month's "GC Connections" continues the coin-and-coffee-can analogy to represent chromatographic separation. This second part explains how the coin-toss experiment does and does not model situations encountered in real-world chromatography.

LCGC 17(11), 1010–1015 (1999).

Articles in this issue

almost 27 years ago

Monoliths as Stationary Phases for Separating Biopolymers – Fourth-Generation Chromatography Sorbents

almost 27 years ago

Automated DNA Fragment Analysis by High Performance Ultrathin-Layer Agarose Gel Electrophoresis

almost 27 years ago

Ion-Exchange Displacement Chromatography: Scale-Up and Displacer Clearance for Recombinant Human Brain-Derived Neurotrophic Factor

almost 27 years ago

Readers' Question – Carryover, Mobile-Phase Temperature, and Column Care

almost 27 years ago

Cleaning Validation

Related to this article

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Figure 2. Split injection used to measure triplicate test of MTBE, hexane, o-xylene, and 1-methylnaphthalene comparing different solvents (methanol, methylene chloride) and column insertion distances starting with 0.5 mm, 5.0 mm, 10.0 mm, 15 mm, and 20.0 mm. Methanol had the best performance at the 5.0 mm insertion distance and methylene chloride looked slightly better at the 0.5 mm. We would still recommend not going below the 5.0 mm manufacturer recommended insertion distance.
Revisiting Column Insertion Distance
Revisiting insertion distance for methanol and methylene chloride in GC analysis and confirming that insertion depth strongly affects response and reproducibility.
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