A Century of Separation Science

The researchers explored ways to simplify retention time calculation across LC–MS and various other chromatographic methods.

A new type of micro-gas chromatography column was fabricated using direct metal laser sintering (DMLS) to circumvent manufacturing issues faced by other micro-GC systems.

This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.

This article discusses several practical examples using bioinert columns, demonstrating their ability to deliver reliable, high-resolution results with precision and reproducibility.

How is the indoor air affecting your health?

Here is some of the most popular content posted on LCGC International this week.