Full characterization of mAbs is vital as the glycan moieties have such a key role in immunogenicity, effector function efficacy and drug clearance. Conventional methods of N-glycan analysis, such as LC–FLD or CE–FLD, can take two to three days to complete a single analysis. The introduction of HPLC-Chip–MS workflow systems have significantly reduced the total analysis time (including on-chip enzymatic reaction, glycan separation and MS analysis) to tens of minutes. This creates a major productivity enhancement for biopharmaceutical analysis.

Biological, Medical, and Clinical Analysis

Liquid Chromatography (LC/HPLC)

The Column-11-19-2010

The advantages of HPLC-Chip-MS workflow over conventional methods of N-glycan analysis