Characterizing Protein-Protein Interactions Using Composition Gradient Multi-angle Light Scattering

January 18, 2010

This article reviews the principles of static light scattering and discusses how new technology complements conventional separation methods to achieve full characterization of proteins and their interactions.

Light scattering, including dynamic and static versions, is the most versatile technology for obtaining information about macromolecules and their properties in solution. Composition gradient multi-angle light scattering (CG-MALS) is a relatively new variant of static light scattering technology, particularly powerful for characterizing macromolecular interactions without labelling or immobilization. This article reviews the principles of static light scattering and discusses how the new CG-MALS technology complements conventional MALS separation methods to achieve full characterization of proteins and their interactions.

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