Chiral Separation of Beta Blocker Pindolol Enantiomers

October 1, 2015

The Application Notebook

Issue 0
Page Number: 26

Pindolol is a non-selective beta-blocker used for treatment of hypertension and angina pectoris. It is applied as a racemate, although only the (S)-form is the active stereoisomer.

YMC offers immobilized chiral phases that can be used either in normal or reversed phase mode as well as in supercritical fluid chromatography (SFC) mode. CHIRAL ART Cellulose-SC is a very versatile phase to separate many chiral substances. YMC have developed two isocratic applications to separate the pindolol enantiomers in normal phase or reversed phase mode. Both methods offer a high resolution. The normal phase application provides more potential for a preparative scale up, while the reversed phase approach separates the enantiomers in a shorter time.

 

Normal Phase Method

Column:   CHIRAL ART Cellulose-SC (250 × 4.6 mm, 5-μm)  
Part No.:   KSC99S05-2546WT
Eluent:   n-hexane/ethanol/diethylamine (40/60/0.1)
Flow rate:1.0 mL/min
Detection:UV at 265 nm
Injection:   10 μL (100 μg/mL)
Temperature:25 °C

 

Reversed Phase Method

Column:   CHIRAL ART Cellulose-SC (250 × 4.6 mm, 5-μm)  
Part No.:   KSC99S05-2546WT
Eluent:   methanol/diethylamine (100/0.1)
Flow rate:1.0 mL/min
Detection:UV at 265 nm
Injection:   10 μL (100 μg/mL)
Temperature:25 °C

 

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