Chlorophyll Removal from Vegetable Extracts using Centrifugal Partition Chromatography

Crude aqueous vegetable leaf extracts often contain a complex mixture of proteins, alkaloids, terpenes, and pigments such as chlorophyll. This application note shows how centrifugal partition chromatography can support chlorophyll remediation by separating unwanted organically soluble compounds from water-soluble protein fractions. The workflow uses LiLiChro’s midiLiLi equipment with a water-based buffer / water-saturated MTBE solvent system, UV detection at 280 nm, and ascending mode operation.