LiLiChro

EGCG, or epigallocatechin gallate, is a high-value green tea polyphenol with strong relevance in nutraceutical, botanical, and natural product research. This application note presents a liquid-liquid chromatography workflow for EGCG purification from green tea polyphenol extract using centrifugal partition chromatography. The note demonstrates how support-free separation can help isolate target polyphenols from complex plant-derived matrices where conventional solid-phase chromatography may face limitations.

Crude aqueous vegetable leaf extracts often contain a complex mixture of proteins, alkaloids, terpenes, and pigments such as chlorophyll. This application note shows how centrifugal partition chromatography can support chlorophyll remediation by separating unwanted organically soluble compounds from water-soluble protein fractions. The workflow uses LiLiChro’s midiLiLi equipment with a water-based buffer / water-saturated MTBE solvent system, UV detection at 280 nm, and ascending mode operation.

Flavoring materials from biological samples often come from complex natural matrices, where target compounds may be present alongside pigments, lipids, proteins, and other interfering components. This application note presents a fast liquid-liquid chromatography workflow for purifying flavoring-related compounds from a biological sample. Using centrifugal partition chromatography, the method demonstrates how support-free separation can help isolate valuable compounds from natural sources without relying on a solid stationary phase.

Ionisable lipids are important in advanced pharmaceutical and biotech workflows, but their hydrophobic character can make purification challenging, especially when water-free conditions are required. This application note demonstrates how centrifugal partition chromatography can be applied to ionisable lipid purification using a support-free liquid-liquid chromatography approach. The workflow shows how CPC can offer an alternative separation strategy for sensitive, hydrophobic compounds where traditional solid-phase chromatography or aqueous methods may create limitations.

Cyclosporine purification can be challenging when crude material contains closely related impurities that require selective fractionation before final polishing. This application note presents a liquid-liquid chromatography workflow using LiLiChro’s miniLiLi CPC equipment for cyclosporine pre-purification. The method demonstrates how centrifugal partition chromatography can support pharmaceutical purification workflows through support-free separation, UV-based fraction collection, and recovery-focused processing.