Choice of Buffer for the Analysis of Basic Peptides in Reversed-Phase HPLC

May 1, 2005
David V. McCalley

Centre for Research in Biomedicine, University of West England.

LCGC Europe

LCGC Europe, LCGC Europe-05-01-2005, Volume 18, Issue 5
Page Number: 290–297

This article examines the results of chromatographic performance when using different buffers. A study of the origins of the differences in peak shape obtained when using varying buffers–additives is presented with regard to the analysis of basic peptides in reversed-phase HPLC.