Choice of Buffer for the Analysis of Basic Peptides in Reversed-Phase HPLC

McCalley,David;

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Choice of Buffer for the Analysis of Basic Peptides in Reversed-Phase HPLC

Published on: May 1, 2005

David V. McCalley

LCGC Europe

LCGC Europe, LCGC Europe-05-01-2005, Volume 18, Issue 5

Pages: 290–297

This article examines the results of chromatographic performance when using different buffers. A study of the origins of the differences in peak shape obtained when using varying buffers–additives is presented with regard to the analysis of basic peptides in reversed-phase HPLC.