Choice of Buffer for the Analysis of Basic Peptides in Reversed-Phase HPLC

The researchers explored ways to simplify retention time calculation across LC–MS and various other chromatographic methods.

Here's a taster of what to look forward to at the 54th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2025), which will be held from Sunday through Thursday, 15–19 June, 2025, in Bruges, Belgium.

This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.

This article discusses several practical examples using bioinert columns, demonstrating their ability to deliver reliable, high-resolution results with precision and reproducibility.

Here is some of the most popular content posted on LCGC International this week.

Hydrophobic deep eutectic solvents have proven useful in extracting non-polar target compounds, though researchers have shown there is room for further development.