David V. McCalley | Authors


Hydrophilic Interaction Liquid Chromatography: An Update

This article is an update on the technique of HILIC and covers recent ideas on the mechanism of separation, and how it may be manipulated to suit the separation of particular sample types. The advantages of HILIC are discussed, and also the actual and perceived disadvantages of the technique and how the latter can be overcome. Some new applications of HILIC for characterization of biopharmaceuticals, where it can even be applied to the separation of intact proteins, and to applications in metabolomics, will be discussed.

Hydrophilic Interaction Chromatography

This article details the principles of hydrophilic interaction chromatography (HILIC) and its complementary selectivity to reversed-phase high performance liquid chromatography (HPLC). Advantages of the technique that result from the use of low-viscosity, high-organic concentration mobile phases will be demonstrated. For example, LC–mass spectrometry (MS) sensitivity is enhanced and higher flow rates and longer columns can be used effectively with such mobile phases in HILIC. Common stationary phases employed in HILIC are reviewed.