Biopharmaceuticals offer great new possibilities and potential in treating various life threatening diseases such as cancer and autoimmune diseases. However, their complexity, resulting from their heterogeneity, represents a great challenge to the analytical chemist and a suite of techniques is required for their characterization and analysis. Reversed phase chromatography, the mainstay of traditional small molecule analysis, is employed at multiple biopharmaceutical levels due to its versatility, inherent relative efficiency and ease of hyphenation to a mass spectrometer.
Biopharmaceuticals offer great new possibilities and potential in treating various life threatening diseases such as cancer and autoimmune diseases. However, their complexity, resulting from their heterogeneity, represents a great challenge to the analytical chemist and a suite of techniques is required for their characterization and analysis. Reversed phase chromatography, the mainstay of traditional small molecule analysis, is employed at multiple biopharmaceutical levels due to its versatility, inherent relative efficiency and ease of hyphenation to a mass spectrometer. However, these separations often rely on finely balanced critical parameters being correctly optimized. We shall interrogate these critical parameters at each level and discuss best practices for implementation and optimization. The considerable increase in glycosylated biopharmaceuticals and the development of biosimilars and biobetters has led to much greater interest in the type and degree of glycosylation. We will review not only the popularity behind glycosylated biopharmaceuticals but where the heterogeneity comes from and how glycans can be effectively liberated, characterized and analyzed using HILIC.
Presented by Dr. Koen Sandra (The Research Institute of Chromatography, RIC) and
Scott Fletcher (Technical Business Development Manager, Crawford Scientific).
Key Learning Objectives:
Who Should Watch:
Watch the full webcast at www.chromacademy.com/Biopharmaceutical-Analysis-Reversed-Phase-HILIC.html.
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