It has been revealed that the collaboration between Thermo Fisher Scientific?s Biomarker Research Initiatives in Mass Spectrometry (BRIMS) Center and three organizations will be extended.
It has been revealed that the collaboration between Thermo Fisher Scientific’s Biomarker Research Initiatives in Mass Spectrometry (BRIMS) Center and three organizations will be extended.
Over the last couple of years, the BRIMS Center, George Mason University’s Center for Applied Proteomics and Molecular Medicine (CAPMM), Johns Hopkins University and Toronto’s University Health Network (UHN) have collaborated to speed up protein biomarker identification for cancer and other diseases. The results of this collaboration were recently demonstrated at the US Human Proteome Organization 2010 meeting in Denver, Colorado, USA.
To address the challenges set out by biomarker researchers seeking to independently validate hypothetical biomarkers and routine mass spectrometry-based quantitative assays suited for clinical research, the BRIMS Center developed targeted SRM-based quantitative mass spectrometry workflows that allow rapid assay development and cross validation of biomarker assays across the network of laboratories. Each of the collaborators uses these biomarkers and assays to perform research on identical Thermo Scientific TSQ Quantum Ultra triple quadrupole mass spectrometry platforms.
The director of the BRIMS Center, Mary Lopez, stated, “Working together, the BRIMS Center and the collaborators demonstrated an instrument platform and an end-to-end workflow from sample preparation through data analysis that are easily and reproducibly transferred from laboratory to laboratory. By continuing our work with these leading research groups, we can extend their ground-breaking research by continuing to apply the SRM-based workflow to previously validated clinical biomarkers.”
This study is reportedly the first inter-lab study to demonstrate reproducible determination of protein abundance in a complex matrix such as blood.
For more information visit Thermo’s website at www.thermo.com/proteomics
This story originally appeared in The Column. Click here to view that issue.
GC–TOF-MS Finds 250 Volatile Compounds in E-Cigarette Liquids
November 1st 2024A study has used gas chromatography coupled to a time-of-flight mass spectrometer to build an electron ionization mass spectra database of more than 250 chemicals classified as either volatile or semi-volatile compounds. An additional, confirmatory layer of liquid chromatography–mass spectrometry analysis was subsequently performed.
AI and GenAI Applications to Help Optimize Purification and Yield of Antibodies From Plasma
October 31st 2024Deriving antibodies from plasma products involves several steps, typically starting from the collection of plasma and ending with the purification of the desired antibodies. These are: plasma collection; plasma pooling; fractionation; antibody purification; concentration and formulation; quality control; and packaging and storage. This process results in a purified antibody product that can be used for therapeutic purposes, diagnostic tests, or research. Each step is critical to ensure the safety, efficacy, and quality of the final product. Applications of AI/GenAI in many of these steps can significantly help in the optimization of purification and yield of the desired antibodies. Some specific use-cases are: selecting and optimizing plasma units for optimized plasma pooling; GenAI solution for enterprise search on internal knowledge portal; analysing and optimizing production batch profitability, inventory, yields; monitoring production batch key performance indicators for outlier identification; monitoring production equipment to predict maintenance events; and reducing quality control laboratory testing turnaround time.
Multivariate Design of Experiments for Gas Chromatographic Analysis
November 1st 2024Recent advances in green chemistry have made multivariate experimental design popular in sample preparation development. This approach helps reduce the number of measurements and data for evaluation and can be useful for method development in gas chromatography.