A Combination of Liquid Chromatography and Mass Spectrometry for Peptidomic Research

Article

LCGC Europe

LCGC EuropeLCGC Europe-10-01-2004
Volume 17
Issue 10
Pages: 522–529

The guest authors describe the use of LC–MS for the analysis of peptides. They deal with peptide extraction, peptidomic analysis in organisms with and without genomic databases, identification of peptides with conserved N- and C- terminal sequences, and in situ MALDI-TOF peptide profiling.

External Link - article-128470.pdf

Articles in this issue
The Role of the Column in Preparative HPLC
Grandma's Ham
Elevated Temperature HPLC: Principles and Applications to Small Molecules and Biomolecules
A Combination of Liquid Chromatography and Mass Spectrometry for Peptidomic Research
Related Videos
Toby Astill | Image Credit: © Thermo Fisher Scientific
Related Content
Estradiol generic estrogen Prescription Medicine Container with other prescription medication bottles in background | Image Credit: © Sherry Young - stock.adobe.com

LC–ESI-MS/MS Quantifies Estradiol with Reduced Reaction Time

April 23rd 2024
Article

The serum/plasma estradiol is useful for diagnosis, pathology, and therapeutic efficacy monitoring of estrogen-dependent diseases, according to the authors.

Human liver model on doctor's table, close-up. Treatment of hepatitis, cirrhosis and liver cancer | Image Credit: © Peakstock - stock.adobe.com

Traditional Chinese Medicine’s Effects on Liver Cancer Measured Using New UHPLC–QTOF-MS System

April 22nd 2024
Article

Scientists from Anhui University of Chinese Medicine in Hefei, China recently investigated the mechanisms behind what makes huachansu tablets, a type of traditional Chinese medicine (TCM), effective against liver cancer.

Katelyn Michael | Image courtesy of Katelyn Michael

Chromatography and PFAS Degradation Challenges: An Interview with Katelyn Michael

April 22nd 2024
Article

On the occasion of Earth Day, University of Wisconsin-Madison graduate student and chemist Katelyn Michael speaks with LCGC about the methods she has been using to attempt to degrade known PFAS.

Angela I. Calderón is a Gilliland Endowed Professor in the Department of Drug Discovery and Development, Harrison College of Pharmacy, Auburn University. She received her B.S. in Pharmacy from the University of Panama, Panama City, Republic of Panama (1990), MSc. in Pharmacognosy from the University of Illinois at Chicago, Chicago, Illinois under the supervision of Dr. Djaja Djendoel Soejarto and Dr. Cindy K. Angerhofer (1997), and Ph.D. in Pharmacognosy from the University of Lausanne, Lausanne, Switzerland under the guidance of Prof. Kurt Hostettmann (2002). Then, she conducted her postdoctorate on biomedical applications of mass spectrometry at Prof. Richard B. van Breemen’s laboratory at the University of Illinois at Chicago (2006-2008). She joined the faculty at Auburn University College of Pharmacy as an Assistant Professor in August 2008. Dr. Calderón specializes in natural drug products research, specifically applications of mass spectrometry to natural products drug discovery, and quality and safety assessment of botanical dietary supplements. Over the last sixteen years, she has authored 52 publications, 1 US patent, and 5 book chapters. She also has received research funding from agencies that include National Center for Complementary and Integrative Health (NCCIH) and the Office of Dietary Supplements (ODS) of the National Institutes of Health (NIH), American Society of Pharmacognosy, United States Pharmacopeia, Auburn University Research Initiative in Cancer (AURIC), and botanical dietary supplements industry. Dr. Calderón is currently an expert member of the United States Pharmacopeia Botanical Pan-American Expert Panel. She also was a member of the United States Pharmacopeia Botanical Dietary Supplements and Herbal Medicines Expert Committee. Additionally, she was the Section Editor of Pharmacognosy of Combinatorial Chemistry & High Throughput Screening and Pharmaceutical Biology and is a current member of the Advisory Board of Planta Medica She has been grant proposal reviewer for National Institutes of Health (NIH), National Science Foundation (NSF), USA and German Federal Ministry of Education and Research (BMBF) and Project Management Jülich (PtJ) and Deutsche Forschungsgemeinschaft (German Research Foundation), Germany, and International Foundation for Science (IFS), Sweeden.

Angela Calderón Discusses Importance of Sustainable Analysis Techniques

April 22nd 2024
Article

In this interview, Angela Calderón of Auburn University discusses the importance of using more environmentally friendly analysis techniques.

Wonder Powder | Image Credit: © Shimadzu

Chromatography and Art Collide at Shimadzu Exhibit in Milan

April 20th 2024
Article

A 10-day exhibit at Milan Design Week in Italy is drawing to a close, but Shimadzu hopes that visitors have recognized the link between science and technology, and art and design.

Best of the Week: Inside the McLean Group Laboratory, Enhancing Omics Techniques

Best of the Week: Inside the McLean Group Laboratory, Enhancing Omics Techniques

April 19th 2024
Article

Here are the top five articles that the editors of LCGC International published this week.