Elevated Temperature HPLC: Principles and Applications to Small Molecules and Biomolecules

In this article the authors review the use of elevated temperatures in HPLC, and provide examples covering separations of both small molecules and biomolecules. Generic issues of temperature dependence of retention and plate height are discussed, and comparisons are made between temperature gradient and solvent gradient elution. They describe how the use of elevated temperatures allow good chromatographic efficiency to be obtained at flow-rates higher than those optimal at ambient temperature, thus increasing the speed of separation.