Cyclosporine Purification Using Liquid-Liquid Chromatography

Cyclosporine purification can be challenging when crude material contains closely related impurities that require selective fractionation before final polishing. This application note presents a liquid-liquid chromatography workflow using LiLiChro’s miniLiLi CPC equipment for cyclosporine pre-purification. The method demonstrates how centrifugal partition chromatography can support pharmaceutical purification workflows through support-free separation, UV-based fraction collection, and recovery-focused processing.