Determination of Chloramphenicol in Honey with Automated Solid-Phase Extraction and HPLC–MS/MS Detection

December 1, 2017
Johannes Brand, Detlef Lambrecht, Hans Rainer Wollseifen

The Application Notebook

Volume 30, Issue 4

Page Number: 695

A sensitive determination method with automated solid‑phase extraction (SPE) is presented and the recovery rates are compared with manual extraction. The automated extraction was developed with the SPE robot FREESTYLETM SPE module (LCTech GmbH). According to the European Regulation (EC) No 470/2009, CHROMABOND® HLB was applied for SPE (1). A high performance liquid chromatography tandem mass spectrometry (HPLC–MS/MS) method with the biphenyl phase NUCLEODUR® π2 was used for the subsequent analysis.

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