Dynamic Liquid-Phase Extraction of Anti-Inflammatory Drugs in Urine
Researchers at the University of Cordoba (Cordoba, Spain) used dynamic liquid-phase microextraction with an ionic liquid as the acceptor phase to extract six nonsteroidal anti-inflammatory drugs from human urine samples.
Researchers at the University of Cordoba (Cordoba, Spain) used dynamic liquid-phase microextraction with an ionic liquid as the acceptor phase to extract six nonsteroidal anti-inflammatory drugs from human urine samples. The extractant liquid was 1-butyl-3-methylimidazolium hexafluorophosphate. The researchers achieved limits of detection ranging from 38 ng/mL to 70 ng/mL and repeatabilities ranging from 2.1% to 3.8%. The analytical method was optimized with respect to chemical, physical, and hydrodynamic variables. The method was found to be useful for drugs in urine in the concentration range 0.1?10 g/mL.
Study Explores Thin-Film Extraction of Biogenic Amines via HPLC-MS/MS
March 27th 2025Scientists from Tabriz University and the University of Tabriz explored cellulose acetate-UiO-66-COOH as an affordable coating sorbent for thin film extraction of biogenic amines from cheese and alcohol-free beverages using HPLC-MS/MS.
Multi-Step Preparative LC–MS Workflow for Peptide Purification
March 21st 2025This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.
