The separation of polar and hydrophilic compounds can be a challenge. Hydrophilic interaction liquid chromatography has proven to be a solid alternative for such applications.
Hydrophilic interaction liquid chromatography (HILIC) is a powerful technique for the separation of polar and hydrophilic compounds. In HILIC one uses a column with a hydrophilic stationary phase and an eluent with water, buffer and a high ratio of water-miscible organic solvent. Today it is generally settled that the retention mechanism is mainly based on partitioning between a water enriched layer, formed on a polar stationary phase under semi-aqueous conditions and a less polar mobile phase. A typical HILIC application uses 50–95% acetonitrile in an aqueous buffer such as ammonium formate, ammonium acetate and/or their corresponding acids or bases, which all have good solubility in organic solvents. HILIC can be used with many detection techniques, but when combined with evaporative detection techniques, such as electrospray ionization mass spectrometry, evaporative light scattering or charged aerosol detection, it will also enable higher sensitivities. A typical chromatogram and conditions are presented.
Microseparations and Analysis Covered at HPLC 2024
July 23rd 2024At HPLC 2024 in Denver, Colorado, various sessions will be led by experienced scientists, covering different aspects of chromatography techniques and maximizing their effectiveness. Here is some information about tomorrow’s sessions.
Automated LC Method Development: An HTC-18 Interview with Bob Pirok
July 23rd 2024At HTC-18 in Leuven, Executive Editor of LCGC International, Alasdair Matheson, spoke to this year’s winner of the 2024 HTC-18 Innovation Award, Bob Pirok from the University of Amsterdam, about his innovative research on automated LC method development.